Enzyme Linked Assays Essay Example | Topics and Well Written Essays - 1250 words

Enzyme Linked Assays - Essay Example hydrogen donors in equation 2 and 3 is quite low, as a result AH2 can be a variety of compounds, and many different bindings or linkings are possible. In this way antibodies are conjugated to enzymes in order to amplify the signal via the catalytic properties of the enzymes. HRP conjugates are ideally used for ELISA or enzyme- linked immunosorbent assays. HRP has a fast catalytic rate and can generate more products in shorter incubation time, allowing for greater sensitivity. HRP-labeled antihuman IgG can be used to bind to target antigen in an assay system to localize even trace amounts of biomolecules. This biomolecules produce dityrosin bonds by reaction with HRP to produce (Adopted from Rob, P.M. et al. 2002). When used for an assay this will give rise to a reaction like Different types of linked assays and their properties: There are mainly two types of ELISA assays. 1. Sandwich. 2. Competitive. Sandwich: This is also known as two-antibody sandwich ELISA. This is used for determination of antigen concentration in unknown samples. With a purified antigen standard, it can determine absolute amount of antigen in a sample. This requires two antibodies, capture and detection, that bind to epitopes that do not overlap. Two monoclonal antibodies that recognize discrete sites on the antigen or two polyclonal affinity purified antibodies are used. The capture antibody is purified and bound to the solid phase. Antigen is added, and antigen-antibody binding occurs. This creates a sandwich. Quantification of the assay is then made by measuring the amount of labeled second antibody bound to the matrix by the use of a colourimetric substrate (Please see the picture). Competitive: Nonpurified primary antibodies may be... Kay,E., Leland, M. S., and Lew, J. Y., (1967). Peroxidase Isozymes from Horseradish Roots, Ii. Catalytic Properties, The Journal Of Biological Chemistry, Vol. 242, No. 10, Issue Of May 25, Pp. 247lbz473,

TMA02 B300 Part 2 Policy issues Essay Example | Topics and Well Written Essays - 2000 words

TMA02 B300 Part 2 Policy issues - Essay Example The Diamond Model consists of four attributes which are interlinked and interrelated with each other. These four attributes must be present in order to ensure rapid growth and development in the industrial sector. Porter stated that national competitive advantage is based upon the application of smart and prudent strategies. Flexible, agile, and reliable government policies will assist in the creation of advanced industries. Other variables like productivity, quality, knowledge base, innovation, and creativity were identified as enhancing the competitive edge of nations. This research paper will explain the four attributes of the Diamond Model. It will analyze the impact of four attributes on national policy. Previous theories related to industrial development and production focused on the availability of human and material resources. However these theories were considered to be flawed because of their linear and simplistic line of reasoning. Most developing countries have abundant material and human resources, yet they have failed to improve economic standards of living. Porter sought to address these concerns by proposing the theory of competitive advantage. National strategy is based upon the application of smart and prudent methods. Governments should have clear and precise goals which encourage investment and business growth. Porter argued that labor intensive industries lead to the production of low level products and services (Suneja, 2002: Pg 113). Competitive advantage helps in knowledge acquisition, quality, innovation, and productivity. It creates highly efficient and effective industries that can eventually target the international market. Porter assumed that competitive advantage occurs in a systematic and methodical manner. An organization focuses on a single process or attribute in order to develop its capabilities. The systematic

Health and Physical Education Assignment Example | Topics and Well Written Essays - 1750 words

Health and Physical Education - Assignment Example (please circle) Individual Pairs Groups of ____6_______ Teams of____________ What do we do – what are the rules? How is the activity conducted? All groups divide into two groups of three, each going on the far side of one of the lines. Lining up, they take turns hopping like a kangaroo from one side of the play field across the line on the other side, releasing the next student to hop across to the other side. How do we include everyone? All children with the ability to hop would be able to participate, but should the class include a member who does not have the capacity to hop, the following modification can be made to the game: instead of hopping the game could be modified so that a ball is bounced as the child travels the distance or that they merely have to travel the distance, which at this point might be widened, passing an item from one person to the next in relay fashion. In this last form, any student that can move can participate, even, as an example, if they are in a wheelchair. How do we make it safe? Include the link to the most appropriate Curriculum Activity Risk Management Guidelines http://education.qld.gov.au/schools/healthy/ Go to this link and scroll down to sport. Select the category or activity that best represents your activities. You might need more than one category. http://education.qld.gov.au/schools/eppr/health/hlspr012/resources/trampolining.pdf While trampolining is a high risk and Kangaroo is a low risk, the same sort of use of the muscles exists, so some of the same risk factors will be included. Teaching the proper way to jump and how to hop from one point to the next would mitigate most of the risk in the game. Activity two:_____Bat and Ball Games____________________________________________ Organisation: How do we set up the activity? Set up a field similar to baseball with four bases at the corners of a diamond shape, but modified to be shorter distances for the age group that has been selected. Provide two fields of pl ay so that two teams of 6 each can be played at the same time. Two plastic bats and two plastic balls will be needed. How are students organised? (please circle) Individual Pairs Groups ________ Teams of___6_________ What do we do – what are the rules? How is the activity conducted? The modified rules of baseball are introduced with the introduction to running the bases after hitting the ball being at the core of the instruction. Children hit a tossed ball (instructors should pitch the ball) so that the opposing team runs to get the ball and then towards the bases. First one to get to the base is successful and the game continues. In other words, if the child who gets the ball beats the batter to the base, the batter is ‘out’. If the batter gets to the base first, they can continue forward from base to base as other hitters hit, each time over home plate counting as a score. The outfield does not exist in this modified version of the game. How do we include every one? Explain how you might modify this activity to ensure all students can participate. You are to imagine that your class has a child with cerebral palsy and think about the modifications that will be

Come up with one Essay Example | Topics and Well Written Essays - 500 words - 2

Come up with one - Essay Example The initially presented the basic principles of ethical decision making focusing on autonomy, beneficence, justice and nonmaleficence. Other mini-case scenarios were presented; together with an identification of significant points concerning the concept of advance directives. Then, ethics at end of life situations, particularly, euthanasia were expounded, detailing the three interventions: active, passive and assisted. Other ethical issues that were discussed were research ethics and health care rationing. The information presented from this discourse in beneficial in the current research through the credibility posed by the author as an associate professor of the John Hopkins University and thereby has been exposed and directly involved in addressing ethical issues faced by older patients at the end of life stage. The discourse presented balanced arguments detailing the legality of the issues; considering this as an option for patients in pain; and other reasons for promoting and using euthanasia. The article likewise identified the groups opposing the legalization of euthanasia and assisted suicide as coming from both medical and disability rights groups. The contents of the article are thereby useful for the current research in terms of providing a balanced discourse and presenting updated statistics given the current status of its publication (2011). Other valid and authoritative sites were likewise provided for more information on the subject, as deemed

Legal Environment Analysis Essay Example | Topics and Well Written Essays - 1500 words

Legal Environment Analysis - Essay Example London hotels generated daily profit - expressed as income before fixed charges (IBFC) - of 47.75 per available room compared to 57.46 the same month a year earlier." (Hospitality Trends, 2009) Although the trends in the hospitality industry at the levels of the hotel chains are not satisfactory the company is going to be the part of the small firms which comprises 99% of the UK business community. The tourism industry of UK comprises of 127,000 businesses in which 80% of the businesses have a turnover of 250,000 pounds per annum. "The London outlook by business segment has also been revised since the Q4 2007 Hotel Market Outlook report when it was expected that the luxury market would lead the sector in 2008. The model now suggests that the mid-market sector will be the front runner, seeing revPAR growth of 7.3% during the year, before falling back to 4.2% in 2009" (Hotel Market Outlook, 2008). The main source of competitive advantage in services industry is efficient service for th e customers. Supply chain plays an important role in the efficient service provision to the customers. "The goal of a supply chain should be to maximise overall supply chain profitability. Supply chain profitability is the difference between the revenue generated from the customer and the total cost incurred across all stages of the supply chain. Supply chain decisions have a large impact on the success or failure of each firm because they significantly influence both the revenue generated as well as the cost incurred. Successful supply chains manage flows of product, information, and funds to provide a high level of product availability to the customer while keeping costs low." (Chopra & Meindl, 2004; 44) The company will also use the same competitive technique in order to keep abreast with the challenges posed due to the high level of competition in the market. Industry Categories or types of establishment Hotels: These are the most luxurious provision of hospitality services (including conference and banqueting), resort, budget and bed and breakfast. Pubs, bars and nightclubs: These are the managed houses where alcoholic beverages are sold. Contract food service providers: These hospitality companies provide services such as schools or hospital catering provision; food and drink and facilities management at different organizations. They also provide services such as housekeeping linen and security. Hospitality services companies: These companies provide support to the provision of in house food and drink. They can be retailers or local authorities. Restaurants: Restaurants are the most popular category of the hospitality business found in UK. These include the Fast food establishments, cafes and coffee shops, mainstream restaurants and fine dining. The proposed business will belong to the same category. The hospitality business will be a restaurant with Oriental theme. Reason for

Energy Sources Of The Future Essay Example for Free

Energy Sources Of The Future Essay Since Industrial Revolution, the use of fossil fuels has been the main source of power for their extensive use in industries, vehicles and in various scientific discoveries. This is continuing till today especially as some of the energy sources are being used up, but the needs of the current power are on the increase whereas the different sources of the planet continue to deplete. Notwithstanding electricity being generated in the power plants is making extensive use of the fossil fuels. The way human is interfering in the natural process for meeting his varied needs is causing the depletion of the fossil fuels at 50 million times more at the rate, which it is being formed. Coal, Oil and Natural gas are the three forms of energy found very near to the surface of the earth. Coal as a source of energy is being extensively used in United States. Oil is another major source of fossil fuel getting extracted every year in the form of petroleum like gasoline, kerosene, gas oil, lubricating oils, and fuel oils to be used in vehicles. Another form is Natural gas mainly being used is methane and is highly inflammable. All over the world scientists are declaring non renewable form of sources as scarce with the continuous search for the alternative renewable sources of energy like wind, air, sun power, heat etc. These forms of energy sources can be replenished continuously. Renewable sources of energy like solar, wind, biomass etc. are dependent on the solar energy directly or indirectly while on the other hand hydroelectric power is not essentially a renewable source of power because in case it is used in the large scale projects, it can result in ecological damage and irreversible consequences. (Farret Simoes, 2006) In 1991, a national wind resource wind inventory by the United States Department of Energy found that three states of the United States, North Dakota, Kansas and Texas have enough of the wind energy to meet the electricity needs of the whole nation. It was found that wind has much greater capacity to produce electricity than any other form of energy would produce. Much of the advancement made in the designs of the wind turbine since 1991 enabled the turbines to operate at lower wind speeds for harnessing more of the wind energy and for harvesting it at much greater heights. Comparatively very large share of the land area can be utilized for generating wind in very scarce populated wind rich areas like Patagonian region of Argentina, Great Plains of North America, Northwest China, and eastern Siberia. In 2002, it was found that it has comparatively higher rate of generating capacity of around 31, 100 megawatts (electricity forum. com) and is increasing continuously satisfying the needs of 40 million Europeans. Utilization of the wind is cheap, conducive to the environment, available in plenty and best renewable source of power. (Associated Press, 2008) Another form of alternative source of energy being experimented on a large scale is the power of sun. Though rate of solar power striking earth is in a very small fraction yet it provides around 10000 times of total commercial energy used by humans on planet. Solar power is an attractive dominant long-term alternative sustainable source of energy. The benefits are manifold- its availability is limitless, environmental friendly, and can be transmitted to earth from sun without any cost. (National Academy of Engineering, Online) Solar panels constitute most common means for tapping solar energy. Sunlight is converted to electricity through the use of solar cells photovoltaics. No doubt solar panels are used for capturing light energy, scientists are still working for improving methods of harvesting sun’s energy. Stephen Maldonado and his team of Michigan University are working upon the designing those systems that can be used for converting solar energy into chemical bond energy, which in turn can be used for producing electricity. The main disadvantage of solar energy is that it cannot be stored for long period and has to be consumed immediately. To overcome this limitation, they are using plants as model because plants are able to convert solar energy into chemical bond energy for producing chemical fuels so they are hoping to devise that system which is able to store solar energy in the form of chemical bonds for long periods of time. (Steinberg, 2009) Geothermal form of energy may not be as conducive as far as its effects are concerned as compared to solar or wind, but it is also best renewable source of energy for the next generation and is one of the most cheaper forms of energy producing 50 times less quantity of CO2, nitric oxide, and sulfur emissions than other conventional use of fossil fuel power plants with other benefit being not requiring power storage. Geothermal energy is limitless and inexhaustible and â€Å"Geothermal power plants run at 89 to 97 percent uptime, in contrast to 75 to 90 percent uptime being offered from coal and nuclear. † (triplepundit, 2009) There is no doubt of the fact that our future lies in tapping nuclear power responsible for extricating 16 per cent of the world’s electric power. There are around 442 nuclear plants which are under operation in 30 countries with most of them operating in Western Europe and North America. Nuclear power creates no impact on global warming, as their emission level of greenhouse gases is virtually nil. From the uranium mining process to waste disposal including reactor and construction, in the whole of nuclear chain emanates only 2-6 grams of carbon per kilowatt-hour. This is very similar to the wind and solar power. It is a fact that if all over the world 440 nuclear power plants had to be closed down and in place are used the mix of non-nuclear sources, there would be an increase of 600 million tones of carbon every year. Though the nuclear plant is costly affair but it is more feasible if its long terms benefits are seen. (The International Atomic Energy Agency, 2004) Among the other sources of energy Algae is being rendered as an important alternative source of energy to biofuels on account of its higher level of energy contents, and yield per acre and its capacity to grow best in water. Its capacity to grow in water is quite huge. As per U. S. Department of Energy (DOE), algae have a potential capacity for producing 100 times more oil per acre than soybeans. Due it’s a higher content level, oil from algae can get refined into biodiesel, green gasoline, jet fuel or ethanol. Moreover, algae require only water, sunlight and CO2 for its growth. (Donovan Stowe, 2009) Looking at the benefits of the several alternative sources of energy, along with their cost effectiveness and the non pollutant properties, the need of the hour is to put an end to the use of the conventional source of energy and make use of the most cost effective alternative sources of energy. The innovative and technologically advanced use of the gift of nature will create an environment of the fresh air with more comforts for the whole human race. Reference List Associated Press. 2008. Wind energy could fuel future power needs. Retrieved on June 14, 2009 from W.W. W. : http://www. electricityforum. com/news/aug03/windenergy. html Donovan, J. Stowe, N. (2009). Is the Future of Biofuels in Algae? Retrieved on June 14, 2009 from W. W. W. : http://www. renewableenergyworld. com/rea/news/article/2009/06/is-the-future-of-biofuels-in-algae? cmpid=rss Farret, F. A. Simoes, M. G. (2006). Integration of alternative sources of energy. New Jersey: John Wiley and Sons. National Academy of Engineering. 2008. Make solar energy economical. Retrieved on June 14, 2009 from W. W. W. : http://www. engineeringchallenges. org/cms/8996/9082. aspx/

Special Needs Students: Learning Life Skills

Special Needs Students: Learning Life Skills All children must learn life skills at one time or another in their lives. Most average children will not only learn life skills at home but will also learn them at school. Special needs children may not have the ability to learn the normal life skills at school as they are generally segregated from the average students. What are life skills, you ask? According to Wikipedia, Life skills are a set of human skills acquired via teaching or direct experience that are used to handle problems and questions commonly encountered in daily human life. .(Wikipedia.org) Unicef states, Life Skills, this term refers to a large group of psycho-social and interpersonal skills which can help people make informed decisions, communicate effectively, and develop coping and self-management skills that may help them lead a healthy and productive life. Life skills may be directed toward personal actions and actions toward others, as well as actions to change the surrounding environment to make it conduciv e to health. (Unicef.org) Life skills are learned by watching others or being taught by another. Average children learn the life skills needed to become successful adults in society without any problems. Most do not even realize they are learning a life skill at the time. How are special needs children supposed to learn these life skills if they are continually segregated from general education students? Inclusion of special needs students into at least elective style classes to learn life skills is a very important step for education. Segregation of special needs students has always been an issue in the education world. One special educator stated, When I started teaching, it was expected that students with significant special needs would be placed in segregated special education classrooms. I noted at that time that these students didnt have access to the same opportunities as the students who participated in the regular education classrooms. Their curriculum was watered down and did not provide exposure to the real world. Their school environment was isolating, and they had only each other as role models and peers. (Lipsitt, A 1998) In following what she wrote, I see a strong problem with trying to teach a special needs student life skills when the ones they are modeling do not have life skills themselves. This is where inclusion helps to change that problem. Inclusion into elective classrooms is a great first step into getting your special needs students into the mainstream of your school. All students deserve the time to socialize with their peers and to learn how to become social accepted. Elective classrooms are a more relaxed and have a less regulated curriculum than core classes. Elective classes pose a more positive situation for the special needs student to feel more relaxed and not so pressured to fit in academically. It is not just a special needs students that need to learn how to fit in, average students also need to learn how to handle being around a special needs student. As an average student of the 80s I do not remember ever having a special needs student in any of my classes. I am not sure we even had that many special needs students at our school, those that were there, were never seen in the regular areas of the school. Today that is something that needs to be changed. It is time to make things equal for all. Everyone deserves to be treated the same regardless of their condition. A person with a disability is not a disabled person. (Lipsitt, A. 1998) This is so true when it comes to a special needs student. Some special needs students are students that just need a bit more help with comprehension, some are physically not able to get around, but are able to understand what is going on around them. Some special needs students are labeled non-communicative. That is wrong on so many levels. A student that does not communicate by normal means is labeled that way, yet they do communicate just not the way you or I would. Each has their own way of communicating what they need or want. Why should we segregate them because we cant understand what they are saying. Maybe we should be segregated for not understanding, and then maybe we would understand what they go through on a daily basis. Inclusion is such a wonderful solution to such a horrific problem of discrimination. One day I hope to see all students sharing all classrooms. Teachers being able to work together to teach all students, the life skills that are necessary to become very successful adults in society in each ones special way is the goal we are after. River Thames Flood Barrier | Review River Thames Flood Barrier | Review Thames Barrier Flood ABSTRACT This is a paper regarding the Thames Barrier. I am trying to present a report on Thames Barrier which is a kind of barrier on the River Thames for flood control. It was constructed between 1974 and 1984. As situated at Woolwich Reach, this barrier is considered to be a remarkable construction. After its construction it was first used defensively in 1983. After that there was no looking back. From 1983 onwards it has been used over 100 times defensively. Till March 19th, 2008 it has got no competitor in its way. Being the worlds second largest movable flood barrier the Thames Barrier stands with all pride and superiority. In this paper I am going to present some of the important things related to this great construction. EXECUTIVE SUMMARY The Thames Barrier located in the downstream of central London, United Kingdom. It is a magnificently well built mega-structure of the world. The purpose of this barrier is to prevent London being flooded. London in general faces seasonal high tide moving up from the sea all these exceptionally high tides are exacerbated by a storm surge. The Thames Barrier is raised for the duration of the high tide in order to prevent it from getting inside the city. As for the dealings with the low tide the Thames Barrier can be opened and as such it release water flowing down the Thames and in general circumstances backs up behind it. The functionality of this Thames Barrier is very unique and is of great use to the people of London. It has been built across a 523 metre wide over the stretched width of the river, this barrier divides the river into four 61m and two 31m navigable spans. Along with these it also divided it into 4 smaller non-navigable channels between nine concrete piers and two abutments. Huge in structure and worth in construction, this is an amazing barrier ranking itself in the second position. THE REPORT The Thames Barrier is for me a remarkable construction and with its gigantic structure it has saved London for 100 times. In this paper I am going to explore all those aspects of this barrier that has made it world’s second largest barrier. I am also trying to find out how appropriately it has been built and how worth is its construction. The roles played by the government and the engineers and the contractors are also my concern. As the paper proceeds I discover certain things that need some kind of discussion and thus the target of my paper. The Thames Barrier took a span of ten years to get constructed. It was started in 1974 and was finished in the year 1984. Its target was to control and to limit the damages that cause by the seasonal flood of London. It is at the Woolwich Reach and from the day of its launch it has protected London from 1oos of disasters. There are of course some amounts of variations too and that is what the main objective of this paper is. The construction is not only unique but is very innovative among its contemporaries. It is the kind of flood gates that gets across the openings in very stylish circular segments in cross section. The engineering of it is also very remarkable and the operation thus has been constructed in a very circular and rotating manner. The water gets raised by hydraulics. The raising of water causes from the horizontal sill on the riverbed and as such there is the appearance of a steel and concrete barrier to check the tidal water. This act of rising gets elongated further and the ration continues allowing underspill operations to control the upstream levels and a complete 180 degree rotation for maintenance of the water levels. The barrier makes a shield of 40mm thickness and the gates fill with water when submerged. It then let it go empty as the water emerges from the river in a huge tidal form. The construction id full proof as there are additional four central gated of 61 mtrs long and 10. 5 mtrs high; along with two outer gates that are of 31.5mtrs. There are four radial gates of 31,5mtrs long by the riverbanks and are flexible enough to be lowered down. These gates are left opening for the ships and at the same time can be rotated and closed to stop water travelling towards London. There is no doubt to the fact that this barrier is a very strong step towards the protection of the city from flood. TECHNICAL UNCERTAINITIES AND INNOVATION It was Charles Draper who came up with the idea of rotating gates and that is the factor that differentiated Thames Barrier from other contemporary constructions. The trio of Rendel, Palmer and Tritton were the actual engineers behind the construction of this particular unique barrier. The selection of the site was also very important and as such it was decided that Woolwich will be the most suitable site as it is the relative straightness of the banks. It was also discovered that in this site the underlying river chalk will act as a strong support the barrier. The construction was initiated by Costain/Hollandsche Beton Maatschappij/Tarmac Construction consortium. The strategically structured things related to the flood defences were made for 11 miles down river and all these equipments were properly raised and strengthened. The amount that was invested in this whole construction was around  £534 million (at 2001 prices). Added to this budget was the additional  £100 million investment was done specifically for the purpose of river defences. The target was for a span of 1000 years. This was undoubtedly a long term thought and investment. There was a clear understanding of land and water before these declarations are made. All these assessments now seem very irrelevant as the estimations are failing as per the predictions. The main reasons behind these diversifications are the increasing factors of global warming and the simultaneous rise of the sea level. The barrier was made in order to cope with the projected sea level that will continuously keep on rising till 2030–2050. Not only these are the possibilities, but there are the suspicions that whether the barrier will be able to complete its promised term. The technical aspects came into being when there was a high tides forecast in the North Sea. The threat was developed more with the declaration that the high river flows at the tidal limit at Teddington weir is the scientifically proven indicator for the measurement of the water levels that was supposed to exceed 4.87 m in central London. The re was the creation and opening of this estuary from the Teddington. At low tide it is a successful effort that can make greater flow rates. With this increase in the rate of flow the further one goes downstream. In the time of upstream flows there gets the act of creating great reservoirs protecting the flood water from rolling down to the city. POLITICS The political roles as has been played are very dramatic and worth all discussion. It was in 2005, that there came up a very interesting suggestion that to supersede the Thames Barrier. The target was huge and there was the application for a 16 km (10 mi) long barrier across the Thames Estuary. It was a very long extension from Sheerness in Kent to Southend in Essex was. After the completion of this the whole got exposed to the public for wider speculations and popularity. The political agreements came into being after the disastrous flood of February 1953, when the sea level was raised by 2 metres at Southend. The disastrous flood covered an area of 64,750 hectares 24,000 houses, and a major set back came into being with 200 major industrial premises, along with a number of 320 kilo-metres of railway. The affects were tremendous over twelve gasworks and two electric power stations that resulted in a heavy loss to the state as a whole. Depressingly enough there were almost 300 people drowned as well as much livestock. The estimations were done about the higher water levels that were supposed to reach 1 in 50 in a year. All these disastrous aspects and the upcoming threats made the governor get more concerned about the consequences. There came up a much stronger political pressure over the government. As such it appointed a departmental committee, the Waverley Committee, to examine the upcoming threats and the relevant solutions to them. As per the report submitted by the committee in 1954, the implementation of a barrier needs to be examined and has to be applied. Initially various sites we re suggested, but finally it was Woolwich Reach. As the construction proceeded there came up a serious concern from the Port of London Authority. According to this concern there will be a single unobstructed opening of not less than 1,400 feet in the barrier that was supposed to be constructed. This was a very turning point towards the added complications of the designing strategies. However in 1965, there came up another high surge tide and this time the Waverley Committee recommendation had got nowhere and the whole importance lied on the construction of the barrier as soon as possible. COMMUNITY INVOLVEMENT There were heavy sufferances towards the outbreak of these seasonal floods. The more precautions are taken, the quicker the water runs into the sewers and then the rivers, and the faster they rise. The area most affected last summer was Severn Trent. It has been estimated that up to ‘4% of the land in its region is converted to hard surfaces each year’. The floodwater runs off land faster than ever with fewer places to go. There are these assessments made about the queries that whether this critical infrastructure sites have adequate defences. There are many questions coming up towards the success of this particular Thames Barrier. The floods in England are devastating and how far is it possible to make the Thames Barrier secured for the purpose is still a question. According to the inspections made by the BBC, the flood alerts as has been applied in England and Netherlands are not much effective and fears of storm-driven tidal surges have not materialised. According t the latest assessments and findings these alerts however are saving much life than 1953. It was in that year about 2,000 people in the two countries faced the devastating affects of the flood. In 1953, in a span of a night thousands of people were evacuated in England and surge barriers closed at the Dutch port of Rotterdam. The rush was heavy and the losses were severe. There were great losses faced by England. In Germany and Denmark, several oil platforms were closed off the Norwegian coast and gales are forecast. These types of scenarios were like lifelong threats to the people of Britain. As a solution to this there was no other option than the application and dependence over the Thames Barrier. The role as has been played by the government way very vital. The only solution that the government could initiate with is the barrier. It was regarded as the modern solution. It was a joint assessment as has been made by the government and the Greater London Council. A barrier was closed without any possibility of failure which was again much supported by public in general. However the acceptability was not granted by the Hydraulic Research Station assessment. As for them it is very complicate business and will not succeed. Against all the opposition the Thames Barrier still stands very firm and is protecting London with all its efforts. There came up this Barrier Act that holds the responsibility for the barrier within the government. It was thus was readily accepted by the Ministry of Agriculture, Fisheries and Food (MAFF). As it was more concerned about the flooding upon the farming section the approval was due. As consequence to this there was a government grant of 75 per cent that was obtained under MAFF. Added to this was the contribution made by the Ministry of Housing and Local Government, the alternative possibility, would have had difficulty in allocating a grant for anything over 40 per cent of the project budget. If the flood in London gets neglected than there are the possibility of thousands of homes, shops, factories, businesses and buildings would be affected. The monetary and financial set backs are sure to happen. If all these get activated than there could be such instances that will make London take moths in functioning again. The financial cost of a major flood could be enormous, possibly topping  £30,000m without counting the cost in human suffering and potential loss of life. SCHEDULE DURATION AND URGENCY After 1953, there were regular assessment being made in order to check the timing and the particular season for the tidal affects. In accordance to the declarations made there were proper evacuations and proper resettlements of the community. The role as played by Thames Barrier here is that it is the protecting shield for the same and there is no such way to make London safer. This barrier needs regular maintenance and that has been well bestowed. Global warming is the main cause behind all these disasters. Even the Thames Barrier which was supposed to stand or 1000 years is hardly going to meet the deadline. The drastic change in the climate has made things tougher. People are more concerned about the threats and their property than anything else. These are many agencies and corporations who are working for the same. According to the assessment as forwarded by the UK Environment Agency; there supposed to have extreme danger to life and property if the Thames Barrier is not maintained well enough. FINANCIAL, LEGAL AND CONTRACTUAL MATTERS The financial cost of a major flood has got the topping of an estimated amount of £30,000m without counting the cost in human suffering. This also excludes the potential losses that had happened. The solution was towards the construction of the Thames Barrier. It was a huge construction and as such was very keenly associated with the works of highly sophisticated engineering. As per the estimations the final cost of the flood defences within the Environment Agencys Thames Region was approximately  £535 million. From the contributions of the taxpayers the expenditure was met by 75% of the approved costs and ratepayers were responsible for the remainder. As undertaken by GLC Department of Public Health Engineering the programme got multifaceted exposure and support from all squares of the population. There were these trio of consultant engineers Rendel,Palmer and Tritton who were in charge of the whole responsibility. All expenditure on which grant aid was claimed was subject to a further detailed check by the engineers and accountants of the Ministry of Agriculture, Fisheries and Food. As a condition of grant aid, all variations over  £50,000 had to have prior approval by the ministry. There was an estimation that states that over 80 staff operate and maintain the Barrier and the associated flood defences. The declarations for any threat could be made before 36 hours in advance and as such the evacuation can happen. However the risks are less due to the Thames Barrier and as such there was hardly any matter to get the threat. The tidal flood risk to London must be seen in the context of the whole estuary or else the Thames Barrier will be a result of heavier investment losses. The tidal Thames seems to be higher than the Thames Barrier and as such the re needs to be some assumptions made over the sustainability of the Barrier. As when it comes to the planning for the future there comes up the negotiation between the Environment Agency, government, local authorities, environmental organisations and others. The objective that all these organisational units hold is to provide long term-answers on flood risk management in the greater Thames Estuary. A severe flood in London most of the time affects the central part of the London. The causes are devastating and the damages were made by the river. The attention should also be given to the fresh water and sewer systems and needs to mend them for the disrupt power, gas, telephone and vital data services. This results n heavy financial losses and as such there are many things that needs to be given extra attentions. CONCLUSION Sir Hermann Bondi, an eminent mathematician, astronomer and government scientist made a revision over the flood threat in 1966. According to the assessment as forwarded by Professor Bondi, there will be a serious flood in London. He further added that this flood would be A disaster of [a] singular and immense kind and that it must not be allowed, particularly since the ability to prevent it existed’. His idea was for the application of a containerisation that was supposed to be reduced the traffic to the Port of London dramatically. This idea was however not accepted and there came up this mega-structure for the proper and specific measures for the protection of flood. The political awareness led the UK Environment Agency to make the futuristic assessments towards the upcoming threats of the flood in London. According to their assessments, there is sure to be an encounter with an extreme danger to life and property. The areas that it specifies are the parts of Norfolk, Suffolk, Kent and Essex. Not only that the UK Environment Agency had issued eight severe flood alerts for the precautionary measures to happen. Added to these alerts there were the declarations made by the Met Office. It announced there is this tremendous gust of up to 145km/h (90mph) that covered the areas of Orkney and Shetland Islands in Scotland. As a result there was a sufferance of around 500 people. All these people were compelled to spend the night at refuge centres set up at local schools and on the upper floors of their houses. The government was having great concern towards it and the sufferance had no other solution than the Thames Barrier. REFERENCES Environment Agency, n.d., http://www.environment-agency.gov.uk/regions/thames/323150/335688/341764/341785/?version=1lang=_e [retrieved on 17.06.08] Reliability of the Thames Barrier, n.d., http://books.google.co.uk/books?id=0AU5Ics8LL8Cpg=PA208dq=bondi+h+1967+thames+barrierei=sfs4SLGGIpyUywTI_5XqDwsig=2eF6CA7we7I5swksvsQVWuPxJUU#PPA199,M1 [retrieved on 17.06.08] Thames Barrier, n.d., http://www.jasa.net.au/london/thames.htm [retrieved on 17.06.08] The penalties of ignoring the risks, n.d., http://www.environment-agency.gov.uk/regions/thames/323150/335688/341764/341770/?lang=_e[retrieved on 17.06.08] RECOMMENDATIONS London By Sarah Johnstone, Tom Masters, Published 2006, Lonely Planet,London (England),ISBN:1740598318